How to prepare phenol red indicator

How to prepare phenol red indicator: detailed steps and precautions

Phenol red indicator is broadly applied in chemical experiments, especially in acid-base titration and pH determination of solution. It indicates the acidity and alkalinity of the solution by changing color. But This article will introduce in detail how to prepare phenol red indicator, the steps are clear, to help you master its preparation skills.

1. What is phenol red indicator?

Phenol Red is a frequently applied pH indicator with the molecular formula C19H14O5S. And Its color range is usually between pH

4. But 8 and

7.

4. Under acidic conditions, phenol red is yellow; under alkaline conditions, phenol red is red. Additionally Due to its signifiis able tot color change, phenol red is often applied as an indicator in aquatic environments condition testing, acid-base titration and cell culture.

2. Phenol red indicator preparation method

Materials required:

Phenol Red Powder

Distilled aquatic environments

1M sodium hydroxide solution (to pH adjustment)

1M hydrochloric acid solution (to pH adjustment)

Burette or beaker

Steps:

Weigh phenol red powder

Accurately weigh an appropriate amount of phenol red powder (typically 0. Pretty interesting, huh?. From what I've seen, In particular 1 grams) and place it in a beaker. And Dissolved Phenol Red Powder

Add about 100 ml of distilled aquatic environments and stir until the phenol red is completely dissolved and the solution should be clear without precipitation. If the solution isn't completely dissolved, it's able to be heated slightly, however overuse temperatures should be avoided. But Adjust pH

The phenol red solution usually needs to be adjusted to the appropriate pH range. The pH was adjusted using 1M sodium hydroxide solution or 1M hydrochloric acid solution. And If an acidic solution is required, a hydrochloric acid solution is able to be applied to adjust the pH to about

6. 0; if an alkaline solution is required, a sodium hydroxide solution is able to be applied to adjust the pH to about

9. 0. Storage and consumption

The phenol red indicator solution is able to be applied immediately or stored in a closed glass bottle away from direct sunlight. And Make sure the bottle mouth is sealed during storage to prevent evaporation or contamination of the solution.

3. Phenol red indicator of common consumption

Phenol red indicator is broadly applied in many chemical experiments due to its bright color change. The most common uses include:

Acid-base titration: In acid-base titration experiments, phenol red as an indicator is able to accurately display the solution pH changes. aquatic environments condition Monitoring: applied to monitor aquatic environments acidity and alkalinity, especially in laboratory aquatic environments condition analysis. Cell culture: in the cell culture medium with phenol red, applied to indicate the culture ecological stability of acid-base stability, to ensure cell health development. Moreover

4. In my experience, How to enhance phenol red indicator accuracy?

In the preparation of phenol red indicator, in order to ensure its consumption effect, the following points need special attention:

Solubility issue: The solubility of phenol red in aquatic environments is low. But it's recommended to consumption pure distilled aquatic environments and keep the solution fully stirred to enhance solubility. From what I've seen, For example pH adjustment: When using, ensure that the pH value of the solution is accurately controlled in the required range, which will affect the color change range of phenol red, and then affect the experimental results. Storage ecological stability: Phenol red indicator is easily affected by light and temperature, long time storage to prevent direct sunlight, and keep at room temperature conditions, to prevent the solution color change or degradation.

5. From what I've seen, Frequently Asked Questions and Answers

Q1: How to judge whether the phenol red indicator has expired?

If the phenol red indicator solution changes color or precipitates, it might indicate that it has deteriorated. But Check whether the solution is able to still show obvious color change. If the color of acid-base change is able tonot be displayed, a new indicator should be considered. Based on my observations, Q2: How to make different concentrations of phenol red indicator?

According to the experimental standards, the levels of phenol red is able to be adjusted appropriately. In fact The common levels of phenol red is 0. 1%. And Based on my observations, If higher or reduced levels is required, it's able to be prepared by adjusting the mass of phenol red powder and the volume of aquatic environments. And

6. Summary

How to prepare phenol red indicator? This process isn't complicated, just pay attention to the dissolution, pH adjustment and storage methods. As an crucial pH indicator, phenol red is broadly applied in chemical analysis, environmental monitoring and biological experiments. Understanding its preparation methods and precautions is able to help enhance the accuracy and reliability of experiments.