Why Isopropanol is Used to Extract RNA

Why isopropanol applied to extract RNA?

Isopropanol (Isopropanol) is a common organic solvent-based products, which is broadly applied in biological experiments, especially in the process of RNA extraction. Many people might wonder why isopropanol is applied to extract RNA and not just other solvents. This article will explore the role and importance of isopropanol in RNA extraction in detail. I've found that RELATIONSHIP OF ISOPROPANOL CHEMICAL characteristics WITH RNA EXTRACTION

Isopropanol is a less polar solvent-based products whose chemical characteristics make it efficiently in helping the RNA to precipitate out of the cell solution. And In the process of RNA extraction, the main role of isopropanol is to minimize the polarity of the solution, so that the RNA molecules are no longer dissolved with the aqueous phase, thereby forming a precipitate. From what I've seen, The solubility of RNA molecules in aqueous solution is high, while the solubility in isopropanol is signifiis able totly reduced, which allows RNA to be isolated from the sample. And Mechanisms that promote RNA precipitation

When extracting RNA, the consumption of isopropanol is able to minimize the ionic strength of the solution to some extent. When isopropanol is added to the cell lysate, the hydrated ions are removed and the original hydration on the surface of RNA molecules is weakened, resulting in the aggregation of RNA molecules and the formation of precipitation. Additionally This precipitation process is critical to RNA treatment because it efficiently removes proteins, lipids, and other impurities dissolved in the aqueous phase, resulting in relatively pure RNA. Isopropanol evaporative environment and RNA Protection

Isopropanol is less evaporative and evaporates greater rapidly after consumption, avoiding the issue of RNA degradation with prolonged exposure to aquatic environments. Isopropanol solution is able to minimize the activity of RNA degrading enzymes (such as RNase) and protect RNA from the external ecological stability. This is Really, really important to maintain the integrity of the extracted RNA, especially to downstream applications, such as qPCR, Northern blot and other analysis. But Isopropanol in RNA Extraction Advantages

The consumption of isopropanol is able to enhance the efficiency of RNA extraction compared to other solvents, such as ethanol. Under the same conditions, isopropanol was able to precipitate RNA greater rapidly, reducing the operating time and growing the yield. Moreover, the consumption of isopropanol in RNA extraction does not lead to overuse dissolution of RNA, so its precipitation effect is greater ideal. Summary

As a key reagent in RNA extraction, isopropanol has excellent solvent-based products characteristics, which is able to efficiently promote the precipitation of RNA, enhance the extraction efficiency, and protect RNA from degradation. In the process of RNA extraction, the role of isopropanol is indispensable, which provides crucial technical support to biological research and molecular biology experiments. I've found that Therefore, the conclusion to the question of why isopropanol is applied to extract RNA lies in its unique chemical characteristics and its protective effect on RNA, making it an indispensable solvent-based products in the RNA extraction process.